Characteristics of dibenzothiophene desulfurization by mutant Gordona sp. EID 


Vol. 5,  No. 2, pp. 79-86, Dec.  1999


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  Abstract

Mutant strain EID was developed by treating Gordona sp. CYKSI with ethylmethanesulfone, and the desulfurization characteristics of dibenzothiophene(DBT) by mutant EID was investigated. Strain EID desulfurized DBT to 2-hydroxybiphenyl (2-HBP) by 4S pathway. Desulfurization rate of the strain EID was 4.0 μmolㆍL(-1)ㆍh(-1), while that of the wild type CYKSI was 2.6 μmolㆍL(-1)ㆍh(-1). The effect of glucose concentration supplied as the carbon source on the DBT desulfurization showed that DBT desulfurization rate was enhanced as the glucose concentration increased. Maximum DBT desulfurization rate was 11.1 μmolㆍL(-1)ㆍh(-1) at 2.0 mM DBT concentration. As end-products such as 2-HBP and sulfate concentrations increase, DBT desulfurization activity of the strain EID decreased. When 0.2 mM of 2-HBP was added in the medium, no growth and desulfurization activity was observed. When 0.5 g/L Na2SO4 was simultaneously supplied with DBT, DBT desulfurization rate was 1.4 μmolㆍL(-1)ㆍh(-1).

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  Cite this article

[IEEE Style]

K. YJ, C. KS, S. JH, R. HW, C. YK, "Characteristics of dibenzothiophene desulfurization by mutant Gordona sp. EID," Clean Technology, vol. 5, no. 2, pp. 79-86, 1999. DOI: .

[ACM Style]

Kim YJ, Cho KS, Sung JH, Ryu HW, and Chang YK. 1999. Characteristics of dibenzothiophene desulfurization by mutant Gordona sp. EID. Clean Technology, 5, 2, (1999), 79-86. DOI: .