Biodegradation of Poly(ethylene glycol) using PETase Immobilized on Bacterial Outer Membrane Vesicles 


Vol. 31,  No. 1, pp. 43-50, Mar.  2025
10.7464/ksct.2025.31.1.43


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  Abstract

Microplastics, particularly poly(ethylene terephthalate), have become a significant environmental concern due to their persistence in aquatic ecosystems. This study presents an ecofriendly approach for PET degradation using Vibrio natriegens (Vn)-derived outer membrane vesicles (OMVs) as carriers for immobilized PETase, a PET-degrading enzyme. Vn was engineered to express a membrane-anchored PETase, which was subsequently secreted as part of the OMVs upon cultivation. These PETase-immobilized OMVs (PETase-OMVs) were harvested via membrane filtration. They had a spherical structure with a size of 150 nm, and displayed significant PET degradation activity because they could effectively break down PET microplastics into monomeric units. This study highlights the potential of using OMVs as a versatile and stable platform for enzyme immobilization in order to create an environmentally friendly and sustainable solution to plastic waste in aquatic environments.

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  Cite this article

[IEEE Style]

C. Ratchakorn and S. I. Lim, "Biodegradation of Poly(ethylene glycol) using PETase Immobilized on Bacterial Outer Membrane Vesicles," Clean Technology, vol. 31, no. 1, pp. 43-50, 2025. DOI: 10.7464/ksct.2025.31.1.43.

[ACM Style]

Chokchaiworarat Ratchakorn and Sung In Lim. 2025. Biodegradation of Poly(ethylene glycol) using PETase Immobilized on Bacterial Outer Membrane Vesicles. Clean Technology, 31, 1, (2025), 43-50. DOI: 10.7464/ksct.2025.31.1.43.

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pISSN : 1598 - 9712
eISSN : 2288 - 0690

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