Effects of Cell Wall on the Transformation of Microalgae by a Digital Microfluidic System 


Vol. 21,  No. 2, pp. 90-95, Jun.  2015
10.7464/ksct.2015.21.2.090


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  Abstract

Digital microfluidic electroporation system was used for the transformation of microalgae and we have obtained higher transformation efficiency and viability than that of conventional method. Key parameters of electroporation such as pulse voltage, number, and duration time were systematically investigated for two different microalgal strains with and without cell wall. We have found that cell wall does not always have negative effects on the gene transformation of microalgae. Parallel processing of proposed digital microfluidic electroporation was demonstrated together with on chip culture of microalgae.

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  Cite this article

[IEEE Style]

I. DJ, "Effects of Cell Wall on the Transformation of Microalgae by a Digital Microfluidic System," Clean Technology, vol. 21, no. 2, pp. 90-95, 2015. DOI: 10.7464/ksct.2015.21.2.090.

[ACM Style]

Im DJ. 2015. Effects of Cell Wall on the Transformation of Microalgae by a Digital Microfluidic System. Clean Technology, 21, 2, (2015), 90-95. DOI: 10.7464/ksct.2015.21.2.090.

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